GE AH9539 Manual do Utilizador descarregar pdf (Página 2)

CONTENTS

Components of the Kit .......................................................................................3

Quality Control ....................................................................................................4

Safety Warnings and Precautions ..............................................................4, 26

Introduction .........................................................................................................5

Materials Not Supplied.......................................................................................8

Protocol ...............................................................................................................9

Supplementary Information.............................................................................11

General guidelines .........................................................................................11

Preparation of template DNA.........................................................................12

Cycle conditions and template quantity.........................................................12

Cycling temperatures ..................................................................................... 12

Number of cycles and quantity of template ...................................................13

Designing a new sequencing primer .............................................................13

Sequencing PCR products ............................................................................15

Elimination of compressions ..........................................................................15

Reading farther from or closer to the primer .................................................17

Denaturing gel electrophoresis......................................................................18

Troubleshooting ...............................................................................................20

Control DNA Sequence ....................................................................................22

References ........................................................................................................23

Related Products ..............................................................................................24

Contact Information .........................................................................................25

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GE AH9539 Manual do Utilizador Página 2